Perception of vocoded speech in domestic dogs.

Humans have an impressive ability to comprehend signal-degraded speech; however, the extent to which comprehension of degraded speech relies on human-specific features of speech perception vs. more general cognitive processes is unknown. Since dogs live alongside humans and regularly hear speech, they can be used as a model to differentiate between these possibilities. One often-studied type of degraded speech is noise-vocoded speech (sometimes thought of as cochlear-implant-simulation speech). Noise-vocoded speech is made by dividing the speech signal into frequency bands (channels), identifying the amplitude envelope of each individual band, and then using these envelopes to modulate bands of noise centered over the same frequency regions - the result is a signal with preserved temporal cues, but vastly reduced frequency information. Here, we tested dogs' recognition of familiar words produced in 16-channel vocoded speech. In the first study, dogs heard their names and unfamiliar dogs' names (foils) in vocoded speech as well as natural speech. In the second study, dogs heard 16-channel vocoded speech only. Dogs listened longer to their vocoded name than vocoded foils in both experiments, showing that they can comprehend a 16-channel vocoded version of their name without prior exposure to vocoded speech, and without immediate exposure to the natural-speech version of their name. Dogs' name recognition in the second study was mediated by the number of phonemes in the dogs' name, suggesting that phonological context plays a role in degraded speech comprehension.

Assessing Different Chronic Wasting Disease Training Aids for Use with Detection Dogs.

Chronic wasting disease (CWD) is a highly infectious, fatal prion disease that affects cervid species. One promising method for CWD surveillance is the use of detection dog-handler teams wherein dogs are trained on the volatile organic compound signature of CWD fecal matter. However, using fecal matter from CWD-positive deer poses a biohazard risk; CWD prions can bind to soil particles and remain infectious in contaminated areas for extended periods of time, and it is very difficult to decontaminate the affected areas. One solution is to use noninfectious training aids that can replicate the odor of fecal matter from CWD-positive and CWD-negative deer and are safe to use in the environment. Trained CWD detection dogs' sensitivity and specificity for different training aid materials (cotton, GetXent tubes, and polydimethylsiloxane, or PDMS) incubated with fecal matter from CWD-positive and CWD-negative deer at two different temperatures (21 °C and 37 °C) for three different lengths of time (6 h, 24 h, and 48 h) were evaluated. Cotton incubated at 21 °C for 24 h was identified as the best aid for CWD based on the dogs' performance and practical needs for training aid creation. Implications for CWD detection training and for training aid selection in general are discussed.

A Randomized Cross-Over Study Comparing Cooling Methods for Exercise-Induced Hyperthermia in Working Dogs in Training.

Working dogs are at a high risk of canine non-pyrogenic hyperthermia, a life-threatening condition that can occur due to physical exertion or environmental factors that inhibit dogs' ability to cool themselves. Two frequently recommended cooling methods to reduce body temperature are water immersion and the application of isopropyl alcohol to paw pads. This cross-over study compared the relative efficacy of these methods in 12 working-dogs-in-training with post-exertional heat stress. On each study day, dogs had a physical exam and performed a warm-up exercise followed by sequential recalls in which dogs ran approximately 25 m between two designated handlers for 10 min until they showed multiple signs of heat stress or their core temperature reached 105 °F (40.6 °C). Dogs' temperature and heart rate were collected after each recall. Dogs completed three study days, and each day, randomly received one of three interventions: passive cooling (no intervention), partial water immersion, or isopropyl alcohol. Post-intervention dogs rested for 20 min. Partial water immersion and isopropyl alcohol both cooled dogs more than no intervention, and water immersion cooled dogs more efficiently than isopropyl alcohol. Additionally, the application of isopropyl alcohol raised dogs' heart rates more than water immersion or no intervention, suggesting that the process of applying isopropyl alcohol is potentially stressful to dogs. Thus, partial water immersion is preferred to cool dogs post-exertion due to its more efficient cooling and better tolerance of use.

Functional measurement of canine muscular fitness: refinement and reliability of the Penn Vet Working Dog Center Sprint Test.

Working, sporting, and companion dogs require muscular fitness to perform their daily tasks, competitive activities, and operational functions effectively and with a low risk of injury. There are currently no methods to measure the muscular fitness of dogs who are not debilitated. Sprint performance is highly correlated with muscular fitness in humans, and various sprint assessments are used to measure performance for sporting and tactical athletes. The Penn Vet Working Dog Center Sprint Test (ST) is a 25 m maximal effort sprint from a down position and was developed to be a low-cost measure of muscular fitness suitable for field use. The purpose of this project was to describe the refinements to the ST, detail the performance and measurement protocol, evaluate the measurement inter-rater and intra-rater reliability, characterize the acceleration profile, and explore the inter-day reliability. Both naïve and experienced raters demonstrated excellent intra-rater and inter-rater reliability. The acceleration profile of the dogs in this study was similar to that of average adult human sprinters and demonstrated the role of muscular fitness in performance over this short distance. Finally, a small group of dogs showed moderate inter-day reliability and provided initial performance data to inform future studies. The ST appears to be a reliable measure of canine muscular fitness and could be used to assess performance in healthy dogs and guide the return to sport or work of debilitated dogs.

Canine detection of chronic wasting disease (CWD) in laboratory and field settings.

Chronic wasting disease (CWD) is a fatal transmissible spongiform encephalopathy that affects both free-ranging and farmed cervid species, including mule deer, white-tailed deer, and elk (Odocoileus hemionus, Odocoileus virginianus, and Cervus canadensis). Due to the long incubation period and variability of clinical signs, CWD can expand and spread to new areas before they reach diagnostically detectable levels. Antemortem testing methods currently available can be difficult to obtain and to be applied to the large numbers required for adequate surveillance. However, key volatile biomarkers could be harnessed for non-invasive antemortem surveillance. Detection dogs are the most effective tool currently available for volatile detection; dogs can effectively complete wildlife surveys at rates surpassing that of humans. This study is the first to demonstrate that trained detection dogs can be used as an antemortem test for CWD. First, we trained three dogs to differentiate between CWD-positive and CWD-negative white-tailed deer faeces in a laboratory setting. Dogs spent significantly more time at the positive sample than the negative samples, suggesting that they differentiated between the positive and negative volatile signatures. We then trained the same dogs to search for CWD-positive faecal samples in a more naturalistic field setting. In the field, dogs found 8/11 CWD-positive samples and had an average false detection rate of 13%. These results suggest that dogs can be trained to differentiate CWD-positive faeces from CWD-negative faeces in both laboratory and field settings. Future studies will compare canine accuracy to other antemortem methods, as well as improved canine training methods.

Language preference in the domestic dog (Canis familiaris).

Studies have shown that both cotton-top tamarins as well as rats can discriminate between two languages based on rhythmic cues. This is similar to the capabilities of young infants, who also rely on rhythmic cues to differentiate between languages. However, the animals in these studies did not have long-term language exposure, so these studies did not specifically assess the role of language experience. In this study, we used companion dogs, who have prolonged exposure to human language in their home environment. These dogs came from homes where either English or Spanish was primarily spoken. The dogs were then presented with speech in English and in Spanish in a Headturn Preference Procedure paradigm to examine their language discrimination abilities as well as their language preferences. Dogs successfully discriminated between the two languages. In addition, dogs showed a novelty effect with their language preference such that Spanish-hearing dogs listened longer to English, and English-hearing dogs listened longer to Spanish. It is unclear what particular cue dogs are utilizing to discriminate between the two languages; future studies should explore dogs' utilization of phonological and rhythmic cues for language discrimination.

The Use of Biological Sensors and Instrumental Analysis to Discriminate COVID-19 Odor Signatures.

The spread of SARS-CoV-2, which causes the disease COVID-19, is difficult to control as some positive individuals, capable of transmitting the disease, can be asymptomatic. Thus, it remains critical to generate noninvasive, inexpensive COVID-19 screening systems. Two such methods include detection canines and analytical instrumentation, both of which detect volatile organic compounds associated with SARS-CoV-2. In this study, the performance of trained detection dogs is compared to a noninvasive headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) approach to identifying COVID-19 positive individuals. Five dogs were trained to detect the odor signature associated with COVID-19. They varied in performance, with the two highest-performing dogs averaging 88% sensitivity and 95% specificity over five double-blind tests. The three lowest-performing dogs averaged 46% sensitivity and 87% specificity. The optimized linear discriminant analysis (LDA) model, developed using HS-SPME-GC-MS, displayed a 100% true positive rate and a 100% true negative rate using leave-one-out cross-validation. However, the non-optimized LDA model displayed difficulty in categorizing animal hair-contaminated samples, while animal hair did not impact the dogs' performance. In conclusion, the HS-SPME-GC-MS approach for noninvasive COVID-19 detection more accurately discriminated between COVID-19 positive and COVID-19 negative samples; however, dogs performed better than the computational model when non-ideal samples were presented.

Effects of Intranasal and Oral Bordetella bronchiseptica Vaccination on the Behavioral and Olfactory Capabilities of Detection Dogs.

The bacterium Bordetella bronchiseptica is responsible for serious respiratory disease in dogs, most often associated with 'kennel cough' (canine infectious tracheobronchitis). It is recommended that dogs are vaccinated against the bacterium every 6-12 months, either by oral or intranasal administration. Any impairment of dogs' olfactory capabilities due to medical treatments may impact their efficiency and accuracy in their jobs. This study examined (1) the effect of intranasal and oral vaccines on the olfactory capabilities of detection dogs; as well as (1) effects of the vaccines on canine behavior. Dogs that were vaccinated initially with the oral and 28 days later with intranasal B. bronchiseptica were generally slower to find the target odor than the dogs that were assigned intranasal then oral vaccine. This result prompted a second between-subjects study to further investigate any impact of intranasal administration of the B. bronchiseptica vaccine on the olfactory capabilities of dogs. The intranasal vaccine was of particular interest due to its prevalent use and potential for nasal inflammation leading to decreased olfactory capabilities. Neither odor threshold nor time spent searching for odor were affected by the intranasal vaccine. Behavioral analyses showed that behaviors associated with the dogs' positive and negative motivation affected their time spent finding the target odor; this suggests that behavior should be considered in future studies of olfactory performance.

The role of linguistic experience in the development of the consonant bias.

Consonants and vowels play different roles in speech perception: listeners rely more heavily on consonant information rather than vowel information when distinguishing between words. This reliance on consonants for word identification is the consonant bias Nespor et al. (Ling 2:203-230, 2003). Several factors modulate infants' development of the consonant bias, including fine-grained temporal processing ability and native language exposure [for review, see Nazzi et al. (Curr Direct Psychol Sci 25:291-296, 2016)]. A rat model demonstrated that mature fine-grained temporal processing alone cannot account for consonant bias emergence; linguistic exposure is also necessary Bouchon and Toro (An Cog 22:839-850, 2019). This study tested domestic dogs, who have similarly fine-grained temporal processing but more language exposure than rats, to assess whether a minimal lexicon and small degree of regular linguistic exposure can allow for consonant bias development. Dogs demonstrated a vowel bias rather than a consonant bias, preferring their own name over a vowel-mispronounced version of their name, but not in comparison to a consonant-mispronounced version. This is the pattern seen in young infants Bouchon et al. (Dev Sci 18:587-598, 2015) and rats Bouchon et al. (An Cog 22:839-850, 2019). In a follow-up study, dogs treated a consonant-mispronounced version of their name similarly to their actual name, further suggesting that dogs do not treat consonant differences as meaningful for word identity. These results support the findings from Bouchon and Toro (An Cog 2:839-850, 2019), suggesting that there may be a default preference for vowel information over consonant information when identifying word forms, and that the consonant bias may be a human-exclusive tool for language learning.

The cocktail party effect in the domestic dog (Canis familiaris).

Like humans, canine companions often find themselves in noisy environments, and are expected to respond to human speech despite potential distractors. Such environments pose particular problems for young children, who have limited linguistic knowledge. Here, we examined whether dogs show similar difficulties. We found that dogs prefer their name to a stress-matched foil in quiet conditions, despite hearing it spoken by a novel talker. They continued to prefer their name in the presence of multitalker human speech babble at signal-to-noise levels as low as 0 dB, when their name was the same intensity as the foil. This surpasses the performance of 1-year-old infants, who fail to prefer their name to a foil at 0 dB (Newman in Dev Psychol 41(2):352-362, 2005). Overall, we find better performance at name recognition in dogs that were trained to do tasks for humans, like service dogs, search-and-rescue dogs, and explosives detection dogs. These dogs were of several different breeds, and their tasks were widely different from one another. This suggests that their superior performance may be due to generally more training and better attention. In summary, these results demonstrate that dogs can recognize their name even in relatively difficult levels of multitalker babble, and that dogs who work with humans are especially adept at name recognition in comparison with companion dogs. Future studies will explore the effect of different types of background noise on word recognition in dogs.